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发育生物学
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Generating Primary Fibroblast Cultures from Mouse Ear and Tail Tissues
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发育生物学
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JoVE 杂志 发育生物学
Generating Primary Fibroblast Cultures from Mouse Ear and Tail Tissues

Generating Primary Fibroblast Cultures from Mouse Ear and Tail Tissues

DOI:
10.3791/53565-v

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06:35 min

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January 10, 2016

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1Immunology Program, Department of Microbiology,Yong Loo Lin School of Medicine, National University of Singapore, 2NUS Graduate School for Integrative Sciences and Engineering,National University of Singapore

Chapters

  • 00:05Title
  • 00:42Extraction of Fibroblasts from Ear and Tail Tissues
  • 03:20Cell Culture and Sub-Culturing of Fibroblasts
  • 05:05Results: Fibroblast Culture Hardiness and Purity
  • 06:03Conclusion

Summary

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We describe a simple and quick experimental procedure for generating primary fibroblasts from the ears and tails of mice. The procedure does not require special animal training and can be used for the generation of fibroblast cultures from ears stored at RT for up to 10 days.

Tags

Primary FibroblastMouse EarMouse TailCell IsolationCollagenase DigestionCell StrainerCell SuspensionCell Washing

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