Journal
/
Chemistry
/
Die Auflösung dynamische Kernpolarisation Besetzung für Echtzeit-enzymatische Reaktionsgeschwindigkeit Messungen von NMR
JoVE Journal
Chemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Chemistry
Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR
Please note that all translations are automatically generated. Click here for the English version.

Die Auflösung dynamische Kernpolarisation Besetzung für Echtzeit-enzymatische Reaktionsgeschwindigkeit Messungen von NMR

Deutsch

Automatically Generated

10,454 Views

•

10:54 min

•

February 23, 2016

DOI:

10.3791/53548-v

DOI:
10.3791/53548-v

•

10:54 min
February 23, 2016

•

10 Views
, , , , ,
1LCBPT – UMR8601, Institut de Chimie,Université Paris Descartes, 2Institute of Physics of Biological Systems,EPFL, 3PARCC – INSERM U970,Université Paris Descartes

Summary

Automatically generated

The sensitivity enhancement provided by dissolution dynamic nuclear polarization (DNP) enables following metabolic processes in real time by NMR and MRI. The characteristics and performances of a dedicated dissolution DNP setup designed for study enzymatic reactions are discussed.

Explore More Videos

Artificial IntelligenceDynamic Nuclear PolarizationNMREnzymatic ReactionReal-time MeasurementDissolution DNPPolarizing SolutionCarbon-13TEMPO RadicalCryostatLiquid HeliumSample ContainerLiquid Nitrogen

Read Article

Cite this Article

Balzan, R., Fernandes, L., Comment, A., Pidial, L., Tavitian, B., Vasos, P. R. Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR. J. Vis. Exp. (108), e53548, doi:10.3791/53548 (2016).

Download .ris file

Copy

Share Video

.

Copy