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Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR
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JoVE Journal Chemie
Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR

10,454 Views

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10:54 min

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February 23, 2016

DOI:

10.3791/53548-v

DOI:
10.3791/53548-v

•

10:54 min
February 23, 2016

•

10 Views
, , , , ,
1LCBPT – UMR8601, Institut de Chimie,Université Paris Descartes, 2Institute of Physics of Biological Systems,EPFL, 3PARCC – INSERM U970,Université Paris Descartes

Summary

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The sensitivity enhancement provided by dissolution dynamic nuclear polarization (DNP) enables following metabolic processes in real time by NMR and MRI. The characteristics and performances of a dedicated dissolution DNP setup designed for study enzymatic reactions are discussed.

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Balzan, R., Fernandes, L., Comment, A., Pidial, L., Tavitian, B., Vasos, P. R. Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR. J. Vis. Exp. (108), e53548, doi:10.3791/53548 (2016).

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