ジャーナル
/
化学
/
Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR
JoVE Journal
化学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 化学
Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR

10,454 Views

•

10:54 min

•

February 23, 2016

DOI:

10.3791/53548-v

DOI:
10.3791/53548-v

•

10:54 min
February 23, 2016

•

10 Views
, , , , ,
1LCBPT – UMR8601, Institut de Chimie,Université Paris Descartes, 2Institute of Physics of Biological Systems,EPFL, 3PARCC – INSERM U970,Université Paris Descartes

概要

Automatically generated

The sensitivity enhancement provided by dissolution dynamic nuclear polarization (DNP) enables following metabolic processes in real time by NMR and MRI. The characteristics and performances of a dedicated dissolution DNP setup designed for study enzymatic reactions are discussed.

Explore More Videos

Artificial IntelligenceDynamic Nuclear PolarizationNMREnzymatic ReactionReal-time MeasurementDissolution DNPPolarizing SolutionCarbon-13TEMPO RadicalCryostatLiquid HeliumSample ContainerLiquid Nitrogen

Read Article

Cite this Article

Balzan, R., Fernandes, L., Comment, A., Pidial, L., Tavitian, B., Vasos, P. R. Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR. J. Vis. Exp. (108), e53548, doi:10.3791/53548 (2016).

Download .ris file

Copy

Share Video

.

Copy