Journal
/
Developmental Biology
/
Ex Vivo Chick Serebellar Dilimleri Kültür ve Mekansal Elektroporasyon granül hücre öncülerinin Hedefli
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Ex Vivo Culture of Chick Cerebellar Slices and Spatially Targeted Electroporation of Granule Cell Precursors
Please note that all translations are automatically generated. Click here for the English version.

Ex Vivo Chick Serebellar Dilimleri Kültür ve Mekansal Elektroporasyon granül hücre öncülerinin Hedefli

Türkçe

Automatically Generated

9,137 Views

•

10:02 min

•

December 14, 2015

DOI:

10.3791/53421-v

DOI:
10.3791/53421-v

•

10:02 min
December 14, 2015

•

3 Views
, ,
1MRC Centre of Developmental Neurobiology,King’s College London, 2School of Biological and Chemical Sciences,Queen Mary, University of London

Summary

Automatically generated

Serebellar dış granül tabakası gelişmekte olan beyinde büyük Transit amplifikasyon sitedir. Burada, embriyonik Gün 14 civciv embriyolardan ex vivo elektroporasyon ve serebellar dilim kültürü kullanarak çoğalması zirvesinde bu tabakaya genetik modifikasyon hedeflemek için bir protokol mevcut.

Explore More Videos

Cerebellar SlicesGranule Cell PrecursorsEx Vivo CultureElectroporationCerebellar External Granule LayerNeuronal ProliferationDifferentiationEvo-devoMedulloblastomaGranule Neuron DevelopmentCerebellum DevelopmentEvolutionDisease

Read Article

Cite this Article

Hanzel, M., Wingate, R. J., Butts, T. Ex Vivo Culture of Chick Cerebellar Slices and Spatially Targeted Electroporation of Granule Cell Precursors. J. Vis. Exp. (106), e53421, doi:10.3791/53421 (2015).

Download .ris file

Copy

Share Video

.

Copy