/
/
Ex Vivo Culture of Chick Cerebellar Slices and Spatially Targeted Electroporation of Granule Cell Precursors
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Ex Vivo Culture of Chick Cerebellar Slices and Spatially Targeted Electroporation of Granule Cell Precursors
Kapitel
- 00:05Titel
- 00:55Dissection of E14 Cerebellum
- 03:00Slice Culture of E14 Cerebellum
- 04:21Electroporation of Cerebellum Slices
- 07:08Preparing Cerebellar Slices for Imaging
- 07:58Results: Cerebellar Tissue Maintains Integrity and Proliferation in Culture
- 09:14Conclusion
The cerebellar external granule layer is the site of the largest transit amplification in the developing brain. Here, we present a protocol to target genetic modification to this layer at the peak of proliferation using ex vivo electroporation and culture of cerebellar slices from embryonic Day 14 chick embryos.
