Journal
/
Neuroscience
/
بديل بسيط لحقن المجسم لالدماغ ضربة قاضية محددة من ميرنا
JoVE Journal
Neuroscience
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
A Simple Alternative to Stereotactic Injection for Brain Specific Knockdown of miRNA
Please note that all translations are automatically generated. Click here for the English version.

بديل بسيط لحقن المجسم لالدماغ ضربة قاضية محددة من ميرنا

العربية

Automatically Generated

12,538 Views

•

06:53 min

•

December 26, 2015

DOI:

10.3791/53307-v

DOI:
10.3791/53307-v

•

06:53 min
December 26, 2015

•

6 Views
, , , , , ,
1CSIR-Institute of Genomics and Integrative Biology, New Delhi, India, 2Academy of Scientific and Innovative Research (AcSIR), New Delhi, India

Summary

Automatically generated

MicroRNAs play crucial roles in the brain and are potential targets for modeling neuro-degeneration. However, perturbing miRNA levels is challenging due to the short length of miRNA and inaccessibility of the brain tissue. This video presents a method for antagomir design and brain specific delivery using a neuropeptide in mice.

Explore More Videos

MiRNAMicroRNAAntagomirLNARVGBrain DeliveryStereotactic InjectionNeurodegenerative DiseaseNeuronal Cell DeathGene ExpressionNeurodevelopmentNeuronal Function

Read Article

Cite this Article

Suryawanshi, H., Sarangdhar, M. A., Vij, M., Roshan, R., Singh, V. P., Ganguli, M., Pillai, B. A Simple Alternative to Stereotactic Injection for Brain Specific Knockdown of miRNA. J. Vis. Exp. (106), e53307, doi:10.3791/53307 (2015).

Download .ris file

Copy

Share Video

.

Copy