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A Simple Alternative to Stereotactic Injection for Brain Specific Knockdown of miRNA
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JoVE Revista Neurociencias
A Simple Alternative to Stereotactic Injection for Brain Specific Knockdown of miRNA

A Simple Alternative to Stereotactic Injection for Brain Specific Knockdown of miRNA

12,538 Views

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06:53 min

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December 26, 2015

DOI:

10.3791/53307-v

DOI:
10.3791/53307-v

•

06:53 min
December 26, 2015

•

6 Views
, , , , , ,
1CSIR-Institute of Genomics and Integrative Biology, New Delhi, India, 2Academy of Scientific and Innovative Research (AcSIR), New Delhi, India

Summary

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MicroRNAs play crucial roles in the brain and are potential targets for modeling neuro-degeneration. However, perturbing miRNA levels is challenging due to the short length of miRNA and inaccessibility of the brain tissue. This video presents a method for antagomir design and brain specific delivery using a neuropeptide in mice.

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MiRNAMicroRNAAntagomirLNARVGBrain DeliveryStereotactic InjectionNeurodegenerative DiseaseNeuronal Cell DeathGene ExpressionNeurodevelopmentNeuronal Function

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Cite this Article

Suryawanshi, H., Sarangdhar, M. A., Vij, M., Roshan, R., Singh, V. P., Ganguli, M., Pillai, B. A Simple Alternative to Stereotactic Injection for Brain Specific Knockdown of miRNA. J. Vis. Exp. (106), e53307, doi:10.3791/53307 (2015).

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