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A Simple Alternative to Stereotactic Injection for Brain Specific Knockdown of miRNA
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JoVE Journal 神経科学
A Simple Alternative to Stereotactic Injection for Brain Specific Knockdown of miRNA

A Simple Alternative to Stereotactic Injection for Brain Specific Knockdown of miRNA

DOI:
10.3791/53307-v

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06:53 min

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December 26, 2015

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, , , , , ,
1CSIR-Institute of Genomics and Integrative Biology, New Delhi, India, 2Academy of Scientific and Innovative Research (AcSIR), New Delhi, India

章

  • 00:00標題
  • 01:52Antagomir Design Strategy
  • 02:10Antagomir-peptide Complex Preparation
  • 03:08Tail Vein Injection of Antagomir-peptide Complex
  • 03:29Behavioural Assays: Hind-limb Clasping
  • 03:48Behavioural Assays: Ledge Test
  • 04:03Behavioural Assays: Footprint Assay
  • 04:32Representative Results
  • 06:21Conclusion

概要

自動翻訳

自動翻訳

MicroRNAs play crucial roles in the brain and are potential targets for modeling neuro-degeneration. However, perturbing miRNA levels is challenging due to the short length of miRNA and inaccessibility of the brain tissue. This video presents a method for antagomir design and brain specific delivery using a neuropeptide in mice.

タグ

MiRNAMicroRNAAntagomirLNARVGBrain DeliveryStereotactic InjectionNeurodegenerative DiseaseNeuronal Cell DeathGene ExpressionNeurodevelopmentNeuronal Function

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