Journal
/
Neuroscience
/
De bouw van het Cell gebaseerde neurotransmitter Fluorescent Engineered Reporters (CNiFERs) voor optische detectie van neurotransmitters<em> In Vivo</em
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Construction of Cell-based Neurotransmitter Fluorescent Engineered Reporters (CNiFERs) for Optical Detection of Neurotransmitters In Vivo
Please note that all translations are automatically generated. Click here for the English version.

De bouw van het Cell gebaseerde neurotransmitter Fluorescent Engineered Reporters (CNiFERs) voor optische detectie van neurotransmitters<em> In Vivo</em

DOI:
10.3791/53290-v

•

12:48 min

•

May 12, 2016

•

, , , ,
1Department of Neuroscience,Icahn School of Medicine at Mount Sinai, 2Department of Physics,University of California, San Diego, 3Section of Neurobiology,University of California, San Diego

Chapters

  • 00:05Title
  • 00:56Lentiviral Transduction of HEK293/TN-XXL/Gqi5 Cells
  • 02:49Preparation for FACS and Isolation of the Single CNiFER Clones
  • 05:17CNiFER Implantation into Mouse Cortex
  • 07:52In Vivo Imaging of CNiFER Clones
  • 10:42Results: Examples of Flexstation Analysis, Agonist-induced FRET Response and Dose Response Curves for D2 CNiFER
  • 12:02Conclusion

Summary

Automatic Translation

Automatic Translation

We presenteren een protocol om op cellen gebaseerde neurotransmitter fluorescerende engineered reporters (CNiFERs) te creëren voor de optische detectie van volumetrische neurotransmitter release.

Tags

Keywords: Cell-based Neurotransmitter Fluorescent Engineered Reporters (CNiFERs)Optical DetectionNeurotransmittersIn VivoHEK293 CellsLentivirusFACS AnalysisAgonist CurveGPCRTNXXLFRETFluorometric Plate Reader

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/53290?article_type=v

Retrograde Fluorescerende Labeling Maakt Gerichte Extracellulair Single-unit Opnemen van geïdentificeerde neuronen<em> In vivo</em
check_url/53290?article_type=v

Functionele neuroimaging Met behulp van ultrasone bloed-hersen barrière verstoring en mangaan-enhanced MRI
check_url/53290?article_type=v

Verdund-schedel Corticale Venster Techniek voor<em> In Vivo</em> Optical Coherence Tomography Imaging
check_url/53290?article_type=v

Modeling Astrocytoom Pathogenese<em> In Vitro</em> En<em> In Vivo</em> Met behulp van corticale Astrocyten of neurale stamcellen uit Conditional, genetisch gemanipuleerde muizen
check_url/53290?article_type=v

TIRFM en pH-gevoelige GFP-sondes naar Neurotransmitter Vesicle Dynamics Evalueer in SH-SY5Y Neuroblastoom Cellen: cell imaging en data-analyse
check_url/53290?article_type=v

Simultaan<em> Ex vivo</em> Functioneel testen van Two netvliezen door<em> In vivo</em> Elektroretinogram System
check_url/53290?article_type=v

Optical Monitoring van Living zenuwuiteinde Labeling in haarfollikel Lanceolate Endings van de<em> Ex Vivo</em> Mouse Ear Skin
check_url/53290?article_type=v

Optische coherentie tomografie: Beeldvorming muis retinale Ganglion cellen In Vivo
check_url/53290?article_type=v

Meten van In Vivo veranderingen in extracellulaire Neurotransmitters tijdens natuurlijk belonen van gedrag in vrouwelijke Syrische Hamsters
check_url/53290?article_type=v

In Vivo Controle van de circadiane klok genexpressie in de muis Suprachiasmatic kern met behulp van fluorescentie verslaggevers

Read Article