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In Vivo Monitoring of Circadian Clock Gene Expression in the Mouse Suprachiasmatic Nucleus Using Fluorescence Reporters
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Neuroscience
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JoVE Journal Neuroscience
In Vivo Monitoring of Circadian Clock Gene Expression in the Mouse Suprachiasmatic Nucleus Using Fluorescence Reporters

In Vivo Monitoring of Circadian Clock Gene Expression in the Mouse Suprachiasmatic Nucleus Using Fluorescence Reporters

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8,097 Views

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07:44 min

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July 04, 2018

DOI:

10.3791/56765-v

DOI:
10.3791/56765-v

•

07:44 min
July 04, 2018

•

1 Views
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1PTN Joint Graduate Program, School of Life Sciences,Peking University, 2National Institute of Biological Sciences, Beijing

Summary

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This newly developed fluorescence-based technology enables long-term monitoring of the transcription of circadian clock genes in the suprachiasmatic nucleus (SCN) of freely moving mice in real-time and at a high temporal resolution.

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Cite this Article

Mei, L., Zhan, C., Zhang, E. E. In Vivo Monitoring of Circadian Clock Gene Expression in the Mouse Suprachiasmatic Nucleus Using Fluorescence Reporters. J. Vis. Exp. (137), e56765, doi:10.3791/56765 (2018).

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