Uso de mitocondrias aisladas a partir de cantidades mínimas de ratón músculo esquelético para alta microplacas rendimiento Mediciones respiratorias

The methods presented provide step-by-step instructions for the performance of a collection of microplate based respirometric assays using isolated mitochondria from minimal quantities of mouse skeletal muscle. These assays are able to measure mechanistic changes/adaptations in mitochondrial oxygen consumption in a commonly used animal model.