Journal
/
Developmental Biology
/
ハイスループットマイクロプレート呼吸測定のためのマウス骨格筋の最小量から単離されたミトコンドリアを使用して
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
Using Isolated Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High throughput Microplate Respiratory Measurements
Please note that all translations are automatically generated. Click here for the English version.

ハイスループットマイクロプレート呼吸測定のためのマウス骨格筋の最小量から単離されたミトコンドリアを使用して

日本語

Automatically Generated

13,351 Views

•

10:39 min

•

October 30, 2015

DOI:

10.3791/53216-v

DOI:
10.3791/53216-v

•

10:39 min
October 30, 2015

•

28 Views
, , , , ,
1The Department of Human Nutrition, Foods, and Exercise,Virginia Tech, 2The Metabolic Phenotyping Core,Virginia Tech, 3Seahorse Bioscience

Summary

Automatically generated

The methods presented provide step-by-step instructions for the performance of a collection of microplate based respirometric assays using isolated mitochondria from minimal quantities of mouse skeletal muscle. These assays are able to measure mechanistic changes/adaptations in mitochondrial oxygen consumption in a commonly used animal model.

Explore More Videos

Key Words:Mitochondrial RespirationSkeletal MuscleMouseMicroplateHigh-throughputIsolated MitochondriaRespiratory Measurements

Read Article

Cite this Article

Boutagy, N. E., Rogers, G. W., Pyne, E. S., Ali, M. M., Hulver, M. W., Frisard, M. I. Using Isolated Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High throughput Microplate Respiratory Measurements. J. Vis. Exp. (104), e53216, doi:10.3791/53216 (2015).

Download .ris file

Copy

Share Video

.

Copy