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Developmental Biology

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JoVE Journal Developmental Biology
Using Isolated Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High throughput Microplate Respiratory Measurements
 

Using Isolated Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High throughput Microplate Respiratory Measurements

Article DOI: 10.3791/53216-v • 10:39 min • October 30th, 2015
October 30th, 2015 •
1,2, 3, 1, 1, 1,2, 1,2
1The Department of Human Nutrition, Foods, and Exercise, Virginia Tech, 2The Metabolic Phenotyping Core, Virginia Tech, 3Seahorse Bioscience

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The methods presented provide step-by-step instructions for the performance of a collection of microplate based respirometric assays using isolated mitochondria from minimal quantities of mouse skeletal muscle. These assays are able to measure mechanistic changes/adaptations in mitochondrial oxygen consumption in a commonly used animal model.

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Key Words: Mitochondrial Respiration Skeletal Muscle Mouse Microplate High-throughput Isolated Mitochondria Respiratory Measurements
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