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Brug af Isoleret Mitokondrier fra minimale mængder af Mouse Skeletmuskel for High throughput Mikropladekrav Respiratory Målinger
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Using Isolated Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High throughput Microplate Respiratory Measurements

Brug af Isoleret Mitokondrier fra minimale mængder af Mouse Skeletmuskel for High throughput Mikropladekrav Respiratory Målinger

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13,265 Views

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10:39 min

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October 30, 2015

DOI:

10.3791/53216-v

DOI:
10.3791/53216-v

•

10:39 min
October 30, 2015

•

2 Views
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1The Department of Human Nutrition, Foods, and Exercise,Virginia Tech, 2The Metabolic Phenotyping Core,Virginia Tech, 3Seahorse Bioscience

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Key Words:Mitochondrial RespirationSkeletal MuscleMouseMicroplateHigh-throughputIsolated MitochondriaRespiratory Measurements

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Boutagy, N. E., Rogers, G. W., Pyne, E. S., Ali, M. M., Hulver, M. W., Frisard, M. I. Using Isolated Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High throughput Microplate Respiratory Measurements. J. Vis. Exp. (104), e53216, doi:10.3791/53216 (2015).

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