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16S rRNA Hedefleme Digoksin-etiketli DNA probu kullanılarak Parafine gömülmüş dokular içinde Bakterilerin in situ tespiti
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Immunology and Infection
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JoVE Journal Immunology and Infection
In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA
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16S rRNA Hedefleme Digoksin-etiketli DNA probu kullanılarak Parafine gömülmüş dokular içinde Bakterilerin in situ tespiti

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10,805 Views

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11:15 min

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May 21, 2015

DOI:

10.3791/52836-v

DOI:
10.3791/52836-v

•

11:15 min
May 21, 2015

•

3 Views
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1Department of Oral Microbiology and Immunology,School of Dentistry and Dental Research Institute, Seoul National University

Summary

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Here a method to localize bacteria within paraffin-embedded tissues using DIG-labeled 16S rRNA-targeting DNA probes has been described. This protocol can be applied to study the role of bacteria in various diseases such as periodontitis, cancers, and inflammatory immune diseases.

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In Situ DetectionBacteriaDigoxin-labeled DNA Probe16S RRNAPeriodontitisGingival BiopsiesElectron MicroscopyImmunohistochemistryImmunofluorescenceFluorescent In Situ Hybridization (FISH)Species-specificUniversalBacterial FormsEpitheliaInflammatory InfiltratesBlood VesselsPeriodontitisCancerInflammatory Immune Diseases

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Choi, Y. S., Kim, Y. C., Baek, K. J., Choi, Y. In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA. J. Vis. Exp. (99), e52836, doi:10.3791/52836 (2015).

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