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Semplificato umani neutrofili extracellulari Traps (NET) Isolamento e Manipolazione
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Immunology and Infection
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JoVE Journal Immunology and Infection
Simplified Human Neutrophil Extracellular Traps (NETs) Isolation and Handling
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Semplificato umani neutrofili extracellulari Traps (NET) Isolamento e Manipolazione

italiano

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28,927 Views

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09:43 min

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April 16, 2015

DOI:

10.3791/52687-v

DOI:
10.3791/52687-v

•

09:43 min
April 16, 2015

•

45 Views
, , , ,
1LD MacLean Surgical Research Laboratories, Department of Surgery,McGill University

Summary

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Nella seguente protocollo, si descrive un modo molto semplice per isolare trappole neutrofili extracellulari (NET) da sangue umano intero utilizzando reagenti facilmente disponibili. Abbiamo poi mostrato come i NET isolati possono essere utilizzati in un'adesione test in vitro con cellule tumorali.

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Cite this Article

Najmeh, S., Cools-Lartigue, J., Giannias, B., Spicer, J., Ferri, L. E. Simplified Human Neutrophil Extracellular Traps (NETs) Isolation and Handling. J. Vis. Exp. (98), e52687, doi:10.3791/52687 (2015).

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