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Immunologie et infection
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Simplified Human Neutrophil Extracellular Traps (NETs) Isolation and Handling
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Immunologie et infection
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Journal JoVE Immunologie et infection
Simplified Human Neutrophil Extracellular Traps (NETs) Isolation and Handling

Simplified Human Neutrophil Extracellular Traps (NETs) Isolation and Handling

DOI:
10.3791/52687-v

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09:43 min

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April 16, 2015

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, , , ,
1LD MacLean Surgical Research Laboratories, Department of Surgery,McGill University

Chapitres

  • 00:05Titre
  • 01:14Neutrophil Isolation from Whole Blood
  • 03:38Neutrophil Extracellular Trap (NET) Generation and Isolation
  • 05:24NET-cancer Cell Static Adhesion Assay
  • 08:18Results: Simple Generation and Isolation of NETs from Human Whole Blood
  • 09:12Conclusion

Summary

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In the following protocol, we describe a very simple way to isolate Neutrophil Extracellular traps (NETs) from human whole blood using readily available reagents. We then demonstrate how the isolated NETs can be used in an in vitro adhesion assay with cancer cells.

Tags

Neutrophil Extracellular Traps (NETs)NET IsolationNET HandlingCell-free NET IsolationNET-based AssaysNET Adhesion AssayA549 Lung Cancer CellsPMA StimulationDensity Gradient SeparationDNase1

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