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Simplified Human Neutrophil Extracellular Traps (NETs) Isolation and Handling
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Simplified Human Neutrophil Extracellular Traps (NETs) Isolation and Handling
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April 16, 2015
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Summary
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In the following protocol, we describe a very simple way to isolate Neutrophil Extracellular traps (NETs) from human whole blood using readily available reagents. We then demonstrate how the isolated NETs can be used in an in vitro adhesion assay with cancer cells.
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Najmeh, S., Cools-Lartigue, J., Giannias, B., Spicer, J., Ferri, L. E. Simplified Human Neutrophil Extracellular Traps (NETs) Isolation and Handling. J. Vis. Exp. (98), e52687, doi:10.3791/52687 (2015).
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