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Generation of Lymphocytic Microparticles and Detection of their Proapoptotic Effect on Airway Epithelial Cells
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Immunology and Infection
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JoVE Journal Immunology and Infection
Generation of Lymphocytic Microparticles and Detection of their Proapoptotic Effect on Airway Epithelial Cells

Generation of Lymphocytic Microparticles and Detection of their Proapoptotic Effect on Airway Epithelial Cells

English

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8,843 Views

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09:26 min

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February 20, 2015

DOI:

10.3791/52651-v

DOI:
10.3791/52651-v

•

09:26 min
February 20, 2015

•

2 Views
, , , , , ,
1Departments of Pediatrics and Pharmacology,University of Montréal, 2Department of Pulmonology, Chongqing Southwest Hospital,Third Military Medical University

Summary

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Cell membrane–shed microparticles (MPs) are active biological vesicles that can be isolated and their pathophysiological effects investigated in various models. Here we describe a method for generating MPs derived from T lymphocytes (LMPs) and for demonstrating their proapoptotic effect on airway epithelial cells.

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Yang, C., Xiong, W., Qiu, Q., Tahiri, H., Gagnon, C., Liu, G., Hardy, P. Generation of Lymphocytic Microparticles and Detection of their Proapoptotic Effect on Airway Epithelial Cells. J. Vis. Exp. (96), e52651, doi:10.3791/52651 (2015).

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