/
/
Generation of Lymphocytic Microparticles and Detection of their Proapoptotic Effect on Airway Epithelial Cells
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Generation of Lymphocytic Microparticles and Detection of their Proapoptotic Effect on Airway Epithelial Cells
8,850 Views
•
09:26 min
•
February 20, 2015
DOI:
Summary
Automatically generated
Cell membrane–shed microparticles (MPs) are active biological vesicles that can be isolated and their pathophysiological effects investigated in various models. Here we describe a method for generating MPs derived from T lymphocytes (LMPs) and for demonstrating their proapoptotic effect on airway epithelial cells.
Read Article
Cite this Article
Yang, C., Xiong, W., Qiu, Q., Tahiri, H., Gagnon, C., Liu, G., Hardy, P. Generation of Lymphocytic Microparticles and Detection of their Proapoptotic Effect on Airway Epithelial Cells. J. Vis. Exp. (96), e52651, doi:10.3791/52651 (2015).
Copy