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Generation of Lymphocytic Microparticles and Detection of their Proapoptotic Effect on Airway Epithelial Cells
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JoVE Journal 免疫学と感染
Generation of Lymphocytic Microparticles and Detection of their Proapoptotic Effect on Airway Epithelial Cells

Generation of Lymphocytic Microparticles and Detection of their Proapoptotic Effect on Airway Epithelial Cells

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09:26 min

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February 20, 2015

DOI:

10.3791/52651-v

DOI:
10.3791/52651-v

•

09:26 min
February 20, 2015

•

2 Views
, , , , , ,
1Departments of Pediatrics and Pharmacology,University of Montréal, 2Department of Pulmonology, Chongqing Southwest Hospital,Third Military Medical University

概要

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Cell membrane–shed microparticles (MPs) are active biological vesicles that can be isolated and their pathophysiological effects investigated in various models. Here we describe a method for generating MPs derived from T lymphocytes (LMPs) and for demonstrating their proapoptotic effect on airway epithelial cells.

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Cite this Article

Yang, C., Xiong, W., Qiu, Q., Tahiri, H., Gagnon, C., Liu, G., Hardy, P. Generation of Lymphocytic Microparticles and Detection of their Proapoptotic Effect on Airway Epithelial Cells. J. Vis. Exp. (96), e52651, doi:10.3791/52651 (2015).

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