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March 07, 2015
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Mature adipocytes may represent an abundant source of stem cells through dedifferentiation, which leads to a homogenous population of fibroblast-like cells. Collagenase digestion is used to isolate mature adipocytes from human fat. The goal of our protocol is to obtain multipotent, dedifferentiated fat cells from human mature adipocytes.
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Lessard, J., Côté, J. A., Lapointe, M., Pelletier, M., Nadeau, M., Marceau, S., Biertho, L., Tchernof, A. Generation of Human Adipose Stem Cells through Dedifferentiation of Mature Adipocytes in Ceiling Cultures. J. Vis. Exp. (97), e52485, doi:10.3791/52485 (2015).
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