/
/
Generation of Human Adipose Stem Cells through Dedifferentiation of Mature Adipocytes in Ceiling Cultures
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Generation of Human Adipose Stem Cells through Dedifferentiation of Mature Adipocytes in Ceiling Cultures
11,524 Views
•
07:35 min
•
March 07, 2015
DOI:
Summary
Automatically generated
Mature adipocytes may represent an abundant source of stem cells through dedifferentiation, which leads to a homogenous population of fibroblast-like cells. Collagenase digestion is used to isolate mature adipocytes from human fat. The goal of our protocol is to obtain multipotent, dedifferentiated fat cells from human mature adipocytes.
Read Article
Cite this Article
Lessard, J., Côté, J. A., Lapointe, M., Pelletier, M., Nadeau, M., Marceau, S., Biertho, L., Tchernof, A. Generation of Human Adipose Stem Cells through Dedifferentiation of Mature Adipocytes in Ceiling Cultures. J. Vis. Exp. (97), e52485, doi:10.3791/52485 (2015).
Copy