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Generation of Human Fettgewebestammzellen durch Dedifferenzierung der Reife Adipozyten in Decken Kulturen
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Generation of Human Adipose Stem Cells through Dedifferentiation of Mature Adipocytes in Ceiling Cultures

Generation of Human Fettgewebestammzellen durch Dedifferenzierung der Reife Adipozyten in Decken Kulturen

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07:35 min

March 07, 2015

DOI:

07:35 min
March 07, 2015

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Summary

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Mature adipocytes may represent an abundant source of stem cells through dedifferentiation, which leads to a homogenous population of fibroblast-like cells. Collagenase digestion is used to isolate mature adipocytes from human fat. The goal of our protocol is to obtain multipotent, dedifferentiated fat cells from human mature adipocytes.

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