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Biologia do Desenvolvimento
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Organotypic Slice Cultures for Studies of Postnatal Neurogenesis
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Organotypic Slice Cultures for Studies of Postnatal Neurogenesis

Organotypic Slice Cultures for Studies of Postnatal Neurogenesis

DOI:
10.3791/52353-v

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08:52 min

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March 04, 2015

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, , ,
1Department of Physiology,University of Toronto, 2Institute of Anatomy and Cell Biology, School of Medicine,National Yang-Ming University, 3Department of Education and Research,Taipei City Hospital

Capítulos

  • 00:05Título
  • 01:21Hippocampal Dissection
  • 04:13Feeding and Maintaining Organotypic Slices
  • 04:47Labelling, Fixation and Storage
  • 05:44Sectioning Tissue for Immunohistochemistry
  • 07:30Results: Postnatal Neurogenesis
  • 08:24Conclusion

Summary

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Here we describe a technique for studying hippocampal postnatal neurogenesis using the organotypic slice culture technique. This method allows for in vitro manipulation of adult neurogenesis and allows for the direct application of pharmacological agents to the cultured hippocampus.

Tags

Organotypic Slice CulturePostnatal NeurogenesisHippocampusDentate GyrusPharmacological ManipulationDeveloping Granule NeuronsMaturation ProcessRodent Brain

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