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Entwicklung eines<em> In vitro</em> Modellsystem für die Untersuchung der Wechselwirkung von<em> Equus</em><em> Caballus</em> IgE mit seinen hochaffinen Rezeptors FcsRI
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Immunology and Infection
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JoVE Journal Immunology and Infection
Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor FcεRI
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Entwicklung eines<em> In vitro</em> Modellsystem für die Untersuchung der Wechselwirkung von<em> Equus</em><em> Caballus</em> IgE mit seinen hochaffinen Rezeptors FcsRI

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12,298 Views

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07:31 min

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November 01, 2014

DOI:

10.3791/52222-v

DOI:
10.3791/52222-v

•

07:31 min
November 01, 2014

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1 Views
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1Biological Department,King Abdulaziz University, 2The Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology,The University of Sheffield

Summary

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The current study describes the development and applications of a genetically engineered assay system based on the transfection of rat basophilic leukemia cells with the equine FcεRIα gene. Transfected cells express a functional receptor where the release of mediators of the allergic response can be activated by IgE and antigen.

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Sabban, S., Ye, H., Helm, B. Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor FcεRI. J. Vis. Exp. (93), e52222, doi:10.3791/52222 (2014).

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