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Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor FcεRI
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Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor FcεRI
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November 01, 2014
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The current study describes the development and applications of a genetically engineered assay system based on the transfection of rat basophilic leukemia cells with the equine FcεRIα gene. Transfected cells express a functional receptor where the release of mediators of the allergic response can be activated by IgE and antigen.
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Sabban, S., Ye, H., Helm, B. Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor FcεRI. J. Vis. Exp. (93), e52222, doi:10.3791/52222 (2014).
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