ジャーナル
/
免疫学と感染
/
Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor FcεRI
JoVE Journal
免疫学と感染
Author Produced
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 免疫学と感染
Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor FcεRI

Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor FcεRI

12,294 Views

•

07:31 min

•

November 01, 2014

DOI:

10.3791/52222-v

DOI:
10.3791/52222-v

•

07:31 min
November 01, 2014

•

1 Views
, ,
1Biological Department,King Abdulaziz University, 2The Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology,The University of Sheffield

概要

Automatically generated

The current study describes the development and applications of a genetically engineered assay system based on the transfection of rat basophilic leukemia cells with the equine FcεRIα gene. Transfected cells express a functional receptor where the release of mediators of the allergic response can be activated by IgE and antigen.

Explore More Videos

Equine IgEFcεRIIn Vitro ModelRBL-2H3.1 CellsTransfectionAllergic Responseβ-hexosaminidase ReleaseDiagnostic ToolTherapeutic Intervention

Read Article

Cite this Article

Sabban, S., Ye, H., Helm, B. Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor FcεRI. J. Vis. Exp. (93), e52222, doi:10.3791/52222 (2014).

Download .ris file

Copy

Share Video

.

Copy