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웨스트 나일 바이러스 돌연변이 균주의 감염에에서 MyD88 매개 세포 면역 반응의 생체 외 분석
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Immunology and Infection
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JoVE Journal Immunology and Infection
In Vitro Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection
Please note that all translations are automatically generated. Click here for the English version.

웨스트 나일 바이러스 돌연변이 균주의 감염에에서 MyD88 매개 세포 면역 반응의 생체 외 분석

English

Automatically Generated

8,104 Views

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10:39 min

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November 27, 2014

DOI:

10.3791/52121-v

DOI:
10.3791/52121-v

•

10:39 min
November 27, 2014

•

4 Views
, , , ,
1Department of Microbiology & Immunology,The University of Texas Medical Branch, 2Department of Pathology,The University of Texas Medical Branch, 3Center for Biodefense and Emerging Infectious Diseases, Sealy Center for Vaccine Development,The University of Texas Medical Branch

Summary

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Two flow cytometry-based methods – an in vitro T cell priming assay and intracellular cytokine staining were utilized to measure antigen presenting capacity of dendritic cells and antigen-specific T cell responses to a West Nile virus mutant infection in mice.

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Cite this Article

Xie, G., Whiteman, M. C., Wicker, J. A., Barrett, A. D., Wang, T. In Vitro Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection. J. Vis. Exp. (93), e52121, doi:10.3791/52121 (2014).

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