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In Vitro Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
In Vitro Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection

In Vitro Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection

8,104 Views

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10:39 min

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November 27, 2014

DOI:

10.3791/52121-v

DOI:
10.3791/52121-v

•

10:39 min
November 27, 2014

•

4 Views
, , , ,
1Department of Microbiology & Immunology,The University of Texas Medical Branch, 2Department of Pathology,The University of Texas Medical Branch, 3Center for Biodefense and Emerging Infectious Diseases, Sealy Center for Vaccine Development,The University of Texas Medical Branch

Summary

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Two flow cytometry-based methods – an in vitro T cell priming assay and intracellular cytokine staining were utilized to measure antigen presenting capacity of dendritic cells and antigen-specific T cell responses to a West Nile virus mutant infection in mice.

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Cite this Article

Xie, G., Whiteman, M. C., Wicker, J. A., Barrett, A. D., Wang, T. In Vitro Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection. J. Vis. Exp. (93), e52121, doi:10.3791/52121 (2014).

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