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Hel-celle Patch-clamp Opptak fra Morphologically- og Neurochemically-identifiserte hippocampus interneuroner
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Chapters
- 00:05Title
- 01:46Preparation of Acute-hippocampal Slices
- 03:00Whole Cell Patch-clamp Recording from Fast-spiking Interneurons
- 05:58Extracellular Electrical Stimulation to Evoke GABAB Receptor-mediated Responses
- 06:56Paired Recordings of Synaptically Coupled Fast-spiking INs and CA1 PCs
- 08:42Visualization and Immunocytochemistry of Fast-spiking Interneurons
- 11:30Results: GABAB Receptor-mediated Currents and Presynapitc Inhibition in Identified Hippocampal Parvalbumin Interneurons
- 13:59Conclusion
Kortikale nettverk styres av en liten, men mangfoldig sett av hemmende interneurons. Funksjonell undersøkelse av interneurons krever derfor målrettet opptak og streng identifikasjon. Beskrevet her er en kombinert tilnærming med hel-celle opptak fra én eller postsynaptisk-koblede par av nevroner med intracellulære merking, post-hoc morfologisk og immuncytokjemisk analyse.
Tags
Whole-cell Patch-clampHippocampal InterneuronsGABAergic Inhibitory InterneuronsNeuronal CircuitsPhysiological HeterogeneityMorphological HeterogeneityNeurochemical HeterogeneityTransgenic AnimalsFluorescent ProteinsIntrinsic PropertiesSynaptic PropertiesIntracellular Dye LabelingMorphological AnalysisImmunocytochemical Analysis
