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从Morphologically-和确定的Neurochemically海马的interneurons全细胞膜片钳记录
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Whole-cell Patch-clamp Recordings from Morphologically- and Neurochemically-identified Hippocampal Interneurons
DOI:

14:37 min

September 30, 2014

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Chapters

  • 00:05Title
  • 01:46Preparation of Acute-hippocampal Slices
  • 03:00Whole Cell Patch-clamp Recording from Fast-spiking Interneurons
  • 05:58Extracellular Electrical Stimulation to Evoke GABAB Receptor-mediated Responses
  • 06:56Paired Recordings of Synaptically Coupled Fast-spiking INs and CA1 PCs
  • 08:42Visualization and Immunocytochemistry of Fast-spiking Interneurons
  • 11:30Results: GABAB Receptor-mediated Currents and Presynapitc Inhibition in Identified Hippocampal Parvalbumin Interneurons
  • 13:59Conclusion

Summary

Automatic Translation

皮层网络由一个小的,但不同组抑制性控制。因此的interneurons功能调查需要有针对性的记录和严格的鉴定。这里描述的是一种涉及从单个或突触耦合对神经元细胞内的标记,事后的形态学和免疫细胞化学分析的全细胞记录相结合的方法。

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