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Una enzima de restricción método basado clonación para evaluar la<em> In vitro</em> Capacidad de replicación del VIH-1 subtipo C Gag-MJ4 quiméricos virus
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Restriction Enzyme Based Cloning Method to Assess the In vitro Replication Capacity of HIV-1 Subtype C Gag-MJ4 Chimeric Viruses
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Una enzima de restricción método basado clonación para evaluar la<em> In vitro</em> Capacidad de replicación del VIH-1 subtipo C Gag-MJ4 quiméricos virus

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15,491 Views

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14:23 min

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August 31, 2014

DOI:

10.3791/51506-v

DOI:
10.3791/51506-v

•

14:23 min
August 31, 2014

•

2 Views
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1Emory Vaccine Center at Yerkes National Primate Research Center,Emory University, 2Department of Pathology and Laboratory Medicine,Emory University

Summary

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HIV-1 pathogenesis is defined by both viral characteristics and host genetic factors. Here we describe a robust method that allows for reproducible measurements to assess the impact of the gag gene sequence variation on the in vitro replication capacity of the virus.

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HIV-1Subtype CGagReplication CapacityRestriction Enzyme CloningMJ4ZambiansCellular Immune PressureHLAViral LoadCD4+ T Cell Decline

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Claiborne, D. T., Prince, J. L., Hunter, E. A Restriction Enzyme Based Cloning Method to Assess the In vitro Replication Capacity of HIV-1 Subtype C Gag-MJ4 Chimeric Viruses. J. Vis. Exp. (90), e51506, doi:10.3791/51506 (2014).

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