Journal
/
免疫与感染
/
A Restriction Enzyme Based Cloning Method to Assess the In vitro Replication Capacity of HIV-1 Subtype C Gag-MJ4 Chimeric Viruses
JoVE 杂志
免疫与感染
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 免疫与感染
A Restriction Enzyme Based Cloning Method to Assess the In vitro Replication Capacity of HIV-1 Subtype C Gag-MJ4 Chimeric Viruses

A Restriction Enzyme Based Cloning Method to Assess the In vitro Replication Capacity of HIV-1 Subtype C Gag-MJ4 Chimeric Viruses

15,491 Views

•

14:23 min

•

August 31, 2014

DOI:

10.3791/51506-v

DOI:
10.3791/51506-v

•

14:23 min
August 31, 2014

•

2 Views
, ,
1Emory Vaccine Center at Yerkes National Primate Research Center,Emory University, 2Department of Pathology and Laboratory Medicine,Emory University

Summary

Automatically generated

HIV-1 pathogenesis is defined by both viral characteristics and host genetic factors. Here we describe a robust method that allows for reproducible measurements to assess the impact of the gag gene sequence variation on the in vitro replication capacity of the virus.

Explore More Videos

HIV-1Subtype CGagReplication CapacityRestriction Enzyme CloningMJ4ZambiansCellular Immune PressureHLAViral LoadCD4+ T Cell Decline

Read Article

Cite this Article

Claiborne, D. T., Prince, J. L., Hunter, E. A Restriction Enzyme Based Cloning Method to Assess the In vitro Replication Capacity of HIV-1 Subtype C Gag-MJ4 Chimeric Viruses. J. Vis. Exp. (90), e51506, doi:10.3791/51506 (2014).

Download .ris file

Copy

Share Video

.

Copy