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Immunology and Infection

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JoVE Journal Immunology and Infection
A Restriction Enzyme Based Cloning Method to Assess the In vitro Replication Capacity of HIV-1 Subtype C Gag-MJ4 Chimeric Viruses
 

A Restriction Enzyme Based Cloning Method to Assess the In vitro Replication Capacity of HIV-1 Subtype C Gag-MJ4 Chimeric Viruses

Article DOI: 10.3791/51506-v • 14:23 min • August 31st, 2014
August 31st, 2014 •
*1, *1, 1,2
1Emory Vaccine Center at Yerkes National Primate Research Center, Emory University, 2Department of Pathology and Laboratory Medicine, Emory University
* These authors contributed equally

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HIV-1 pathogenesis is defined by both viral characteristics and host genetic factors. Here we describe a robust method that allows for reproducible measurements to assess the impact of the gag gene sequence variation on the in vitro replication capacity of the virus.

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Restriction Enzyme Based Cloning HIV-1 Subtype C Gag-MJ4 Chimeric Viruses In Vitro Replication Capacity HLA Class I Alleles Conserved Portions Of HIV-1 Genome Cellular Immune Pressure Transmission Of HLA-linked Polymorphisms Reduced Set Point Viral Loads Acute Time Points Subtype C Infected Zambians Proviral Backbone MJ4 Recombination Based Methods Replication Capacity Assessment
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