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High-throughput screening voor Breed-spectrum Chemische Remmers van RNA-virussen
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Immunology and Infection
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JoVE Journal Immunology and Infection
High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses
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High-throughput screening voor Breed-spectrum Chemische Remmers van RNA-virussen

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13,490 Views

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11:34 min

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May 05, 2014

DOI:

10.3791/51222-v

DOI:
10.3791/51222-v

•

11:34 min
May 05, 2014

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6 Views
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1Unité de Génomique Virale et Vaccination, Virology Department,Institut Pasteur, CNRS UMR3569, 2Unité de Chimie et Biocatalyse, Biochemistry and Structural Biology Department,Institut Pasteur, CNRS UMR3523, 3Unité des Interactions Moléculaires Flavivirus-Hôtes, Virology Department,Institut Pasteur

Summary

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In vitro assays om virusreplicatie te meten zijn sterk verbeterd door de ontwikkeling van recombinante RNA-virussen tot expressie luciferase of andere enzymen die in staat bioluminescentie. Hier hebben we detail een high-throughput screening pijplijn dat dergelijke recombinante stammen van mazelen en chikungunya virussen breed-spectrum antivirale middelen te isoleren van chemische bibliotheken combineert.

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High-throughput ScreeningBroad-spectrum Chemical InhibitorsRNA VirusesCellular PathwaysHost Cell FunctionsAntiviral CompoundsRecombinant VirusesReporter EnzymesBioluminescenceLuciferaseMeasles VirusChikungunya VirusCell Culture Assays

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Cite this Article

Lucas-Hourani, M., Munier-Lehmann, H., Helynck, O., Komarova, A., Desprès, P., Tangy, F., Vidalain, P. High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses. J. Vis. Exp. (87), e51222, doi:10.3791/51222 (2014).

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