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Les méthodes de détection quantitative d'anticorps induite par l'activation du complément sur les globules rouges
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Immunology and Infection
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JoVE Journal Immunology and Infection
Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells
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Les méthodes de détection quantitative d'anticorps induite par l'activation du complément sur les globules rouges

30,195 Views

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06:29 min

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January 29, 2014

DOI:

10.3791/51161-v

DOI:
10.3791/51161-v

•

06:29 min
January 29, 2014

•

7 Views
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1Department of Immunopathology, Sanquin Research and Landsteiner Laboratory Academic Medical Center,University of Amsterdam, 2Department of Hematology, Academic Medical Center,University of Amsterdam

Tags

AntibodyComplement ActivationRed Blood CellsRBCAlloantibodiesAutoantibodiesAutoimmune Hemolytic AnemiaAIHACoombs TestHemolytic AssayC3 DepositionC4 DepositionFACS AnalysisQuantitative Hemolytic AssaySpectrophotometryHemoglobin

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Meulenbroek, E. M., Wouters, D., Zeerleder, S. Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells. J. Vis. Exp. (83), e51161, doi:10.3791/51161 (2014).

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