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Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells

Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells

30,234 Views

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06:29 min

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January 29, 2014

DOI:

10.3791/51161-v

DOI:
10.3791/51161-v

•

06:29 min
January 29, 2014

•

8 Views
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1Department of Immunopathology, Sanquin Research and Landsteiner Laboratory Academic Medical Center,University of Amsterdam, 2Department of Hematology, Academic Medical Center,University of Amsterdam

Summary

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Here we describe two assays for measuring complement activation induced by antibodies against red blood cells. The major advantage over the current assays is their quantitative and easy-to-interpret nature.

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Meulenbroek, E. M., Wouters, D., Zeerleder, S. Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells. J. Vis. Exp. (83), e51161, doi:10.3791/51161 (2014).

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