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Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells
JoVE Journal
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Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells

Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells

DOI:
10.3791/51161-v

•

06:29 min

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January 29, 2014

•

, ,
1Department of Immunopathology, Sanquin Research and Landsteiner Laboratory Academic Medical Center,University of Amsterdam, 2Department of Hematology, Academic Medical Center,University of Amsterdam

'Bölümler'

  • 00:05'Başlık'
  • 01:21Complement Deposition Analysis and Quantification of Hemolysis
  • 04:34Results: Quantitative Detection of Antibody-induced Complement Activation on RBCs
  • 06:02Conclusion

Özet

'Otomatik Çeviri'

'Otomatik Çeviri'

Here we describe two assays for measuring complement activation induced by antibodies against red blood cells. The major advantage over the current assays is their quantitative and easy-to-interpret nature.

'Etiketler'

AntibodyComplement ActivationRed Blood CellsRBCAlloantibodiesAutoantibodiesAutoimmune Hemolytic AnemiaAIHACoombs TestHemolytic AssayC3 DepositionC4 DepositionFACS AnalysisQuantitative Hemolytic AssaySpectrophotometryHemoglobin

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