ジャーナル
/
免疫学と感染
/
Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells
JoVE Journal
免疫学と感染
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 免疫学と感染
Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells

Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells

30,234 Views

•

06:29 min

•

January 29, 2014

DOI:

10.3791/51161-v

DOI:
10.3791/51161-v

•

06:29 min
January 29, 2014

•

8 Views
, ,
1Department of Immunopathology, Sanquin Research and Landsteiner Laboratory Academic Medical Center,University of Amsterdam, 2Department of Hematology, Academic Medical Center,University of Amsterdam

概要

Automatically generated

Here we describe two assays for measuring complement activation induced by antibodies against red blood cells. The major advantage over the current assays is their quantitative and easy-to-interpret nature.

Explore More Videos

AntibodyComplement ActivationRed Blood CellsRBCAlloantibodiesAutoantibodiesAutoimmune Hemolytic AnemiaAIHACoombs TestHemolytic AssayC3 DepositionC4 DepositionFACS AnalysisQuantitative Hemolytic AssaySpectrophotometryHemoglobin

Read Article

Cite this Article

Meulenbroek, E. M., Wouters, D., Zeerleder, S. Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells. J. Vis. Exp. (83), e51161, doi:10.3791/51161 (2014).

Download .ris file

Copy

Share Video

.

Copy