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Imaging InlC Secretion to Investigate Cellular Infection by the Bacterial Pathogen Listeria monocytogenes
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JoVE Journal Immunologia e infezioni
Imaging InlC Secretion to Investigate Cellular Infection by the Bacterial Pathogen Listeria monocytogenes

Imaging InlC Secretion to Investigate Cellular Infection by the Bacterial Pathogen Listeria monocytogenes

9,741 Views

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14:05 min

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September 19, 2013

DOI:

10.3791/51043-v

DOI:
10.3791/51043-v

•

14:05 min
September 19, 2013

•

1 Views
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1Unité des Interactions Bactéries Cellules,Pasteur Institute, 2INSERM U604, 3Institut National de la Recherche Agronomique (INRA), USC2020, 4Institute of Biochemistry,ETH Zürich, 5Focal Area Infection Biology, Biozentrum,University of Basel

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Listeria monocytogenes is a Gram positive bacterial pathogen frequently used as a major model for the study of intracellular parasitism. Imaging late L. monocytogenes infection stages within the context of small-interfering RNA screens allows for the global study of cellular pathways required for bacterial infection of target host cells.

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Listeria MonocytogenesBacterial PathogenIntracellular ParasitismCellular Immune ResponseInlCFluorescent LabelingMicroscopyHigh-throughput SiRNA ScreeningCellular Signaling Pathways

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Cite this Article

Kühbacher, A., Gouin, E., Mercer, J., Emmenlauer, M., Dehio, C., Cossart, P., Pizarro-Cerdá, J. Imaging InlC Secretion to Investigate Cellular Infection by the Bacterial Pathogen Listeria monocytogenes. J. Vis. Exp. (79), e51043, doi:10.3791/51043 (2013).

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