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Overvåge ændringer i Membrane polaritet, Membrane Integritet, og intracellulære Ion Koncentrationerne i<em> Streptococcus pneumoniae</em> Brug fluorescerende farvestoffer
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1Department of Microbiology and Immunology,University at Buffalo, State University of New York, 2Witebsky Center for Microbial Pathogenesis and Immunology,University at Buffalo, State University of New York, 3New York State Center of Excellence in Bioinformatics and Life Sciences,University at Buffalo, State University of New York
Chapters
- 00:05Title
- 01:15Detecting Membrane Depolarization and Rupture
- 02:58Detecting Changes in Intracellular Ca2+ or K+ Concentrations
- 05:31Detecting Changes in Intracellular pH
- 08:56Changes in Membrane Polarity, Membrane Integrity, and Intracellular Ion Concentrations
- 10:49Conclusion
I modsætning til det, der ses for eukaryoter, er der en mangel på undersøgelser, der detaljeret membrandepolarisering og ionkoncentration ændringer i bakterier, primært som deres lille størrelse gør konventionelle målemetoder vanskelig. Her har vi detalje protokoller for overvågning af sådanne begivenheder i den betydelige Gram-positive patogen Streptococcus pneumoniae udnytte fluorescens teknikker.
