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ניטור שינויים בממברנה קוטביות, ממברנה יושרה, וריכוזי יון תאיים ב<em> Streptococcus pneumoniae</em> שימוש צבעי ניאון
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Immunology and Infection
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JoVE Journal Immunology and Infection
Monitoring Changes in Membrane Polarity, Membrane Integrity, and Intracellular Ion Concentrations in Streptococcus pneumoniae Using Fluorescent Dyes
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ניטור שינויים בממברנה קוטביות, ממברנה יושרה, וריכוזי יון תאיים ב<em> Streptococcus pneumoniae</em> שימוש צבעי ניאון

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11:17 min

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February 17, 2014

DOI:

10.3791/51008-v

DOI:
10.3791/51008-v

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11:17 min
February 17, 2014

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1Department of Microbiology and Immunology,University at Buffalo, State University of New York, 2Witebsky Center for Microbial Pathogenesis and Immunology,University at Buffalo, State University of New York, 3New York State Center of Excellence in Bioinformatics and Life Sciences,University at Buffalo, State University of New York

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Membrane PolarityMembrane IntegrityIntracellular Ion ConcentrationsFluorescent DyesStreptococcus PneumoniaeMembrane DepolarizationIon FluxesFluorescent IndicatorsDiBAC4(3)Propidium IodideFura-2PBFIBCECFFluorescence Detection

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Clementi, E. A., Marks, L. R., Roche-Håkansson, H., Håkansson, A. P. Monitoring Changes in Membrane Polarity, Membrane Integrity, and Intracellular Ion Concentrations in Streptococcus pneumoniae Using Fluorescent Dyes. J. Vis. Exp. (84), e51008, doi:10.3791/51008 (2014).

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