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Monitoring Changes in Membrane Polarity, Membrane Integrity, and Intracellular Ion Concentrations in Streptococcus pneumoniae Using Fluorescent Dyes
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Monitoring Changes in Membrane Polarity, Membrane Integrity, and Intracellular Ion Concentrations in Streptococcus pneumoniae Using Fluorescent Dyes
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11:17 min
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February 17, 2014
DOI:
1Department of Microbiology and Immunology,University at Buffalo, State University of New York, 2Witebsky Center for Microbial Pathogenesis and Immunology,University at Buffalo, State University of New York, 3New York State Center of Excellence in Bioinformatics and Life Sciences,University at Buffalo, State University of New York
Summary
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Unlike that seen for eukaryotes, there is a paucity of studies that detail membrane depolarization and ion concentration changes in bacteria, primarily as their small size makes conventional methods of measurement difficult. Here, we detail protocols for monitoring such events in the significant Gram-positive pathogen Streptococcus pneumoniae utilizing fluorescence techniques.
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Clementi, E. A., Marks, L. R., Roche-Håkansson, H., Håkansson, A. P. Monitoring Changes in Membrane Polarity, Membrane Integrity, and Intracellular Ion Concentrations in Streptococcus pneumoniae Using Fluorescent Dyes. J. Vis. Exp. (84), e51008, doi:10.3791/51008 (2014).
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