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器官型脳スライスと癌細胞の3Dスフェロイドとの共培養システム
JoVE Journal
Medicine
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JoVE Journal Medicine
Coculture System with an Organotypic Brain Slice and 3D Spheroid of Carcinoma Cells
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器官型脳スライスと癌細胞の3Dスフェロイドとの共培養システム

日本語

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20,302 Views

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07:48 min

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October 09, 2013

DOI:

10.3791/50881-v

DOI:
10.3791/50881-v

•

07:48 min
October 09, 2013

•

16 Views
, , , , ,
1Department of Hematology and Oncology,University of Göttingen, 2Institute of Neuropathology,University of Göttingen, 3Institute of Anatomy and Cellbiology,University of Halle

Summary

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癌細胞との器官型脳切片の共培養は、脳組織の癌細胞浸潤の過程で蛍光だけでなく、明視野（ビデオ）顕微鏡によって形態学的変化を可視化することができます。このモデル系は、細胞の交換や補充的なアプローチを可能にし、操作及び分析を幅広く提供しています。

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Cite this Article

Chuang, H., Lohaus, R., Hanisch, U., Binder, C., Dehghani, F., Pukrop, T. Coculture System with an Organotypic Brain Slice and 3D Spheroid of Carcinoma Cells. J. Vis. Exp. (80), e50881, doi:10.3791/50881 (2013).

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