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Coculture System with an Organotypic Brain Slice and 3D Spheroid of Carcinoma Cells
JoVE 杂志
医学
This content is Free Access.
JoVE 杂志 医学
Coculture System with an Organotypic Brain Slice and 3D Spheroid of Carcinoma Cells

Coculture System with an Organotypic Brain Slice and 3D Spheroid of Carcinoma Cells

DOI:
10.3791/50881-v

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07:48 min

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October 09, 2013

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, , , , ,
1Department of Hematology and Oncology,University of Göttingen, 2Institute of Neuropathology,University of Göttingen, 3Institute of Anatomy and Cellbiology,University of Halle

Chapters

  • 00:05Title
  • 01:41Organotypic Brain Slice Coculturing
  • 04:11Immunofluorescence and Imaging of Astrocytes and Microglia
  • 05:42Representative Coculturing Results
  • 07:11Conclusion

Summary

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The organotypic brain slice coculture with carcinoma cells enables visualizing morphological changes by fluorescence as well as bright field (video) microscopy during the process of carcinoma cell invasion of brain tissue. This model system also allows for cell exchange and replenishment approaches and offers a wide variety of manipulations and analyses.

Tags

CocultureOrganotypic Brain Slice3D SpheroidCarcinoma CellsCerebral MetastasisTumor MicroenvironmentTumor-associated Macrophages (TAM)AstrocytesIn Vitro ModelBrain ColonizationGlial ReactionsMolecular Analysis

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