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Coculture System with an Organotypic Brain Slice and 3D Spheroid of Carcinoma Cells
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Medicine
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JoVE Journal Medicine
Coculture System with an Organotypic Brain Slice and 3D Spheroid of Carcinoma Cells

Coculture System with an Organotypic Brain Slice and 3D Spheroid of Carcinoma Cells

English

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20,301 Views

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07:48 min

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October 09, 2013

DOI:

10.3791/50881-v

DOI:
10.3791/50881-v

•

07:48 min
October 09, 2013

•

16 Views
, , , , ,
1Department of Hematology and Oncology,University of Göttingen, 2Institute of Neuropathology,University of Göttingen, 3Institute of Anatomy and Cellbiology,University of Halle

Summary

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The organotypic brain slice coculture with carcinoma cells enables visualizing morphological changes by fluorescence as well as bright field (video) microscopy during the process of carcinoma cell invasion of brain tissue. This model system also allows for cell exchange and replenishment approaches and offers a wide variety of manipulations and analyses.

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Cite this Article

Chuang, H., Lohaus, R., Hanisch, U., Binder, C., Dehghani, F., Pukrop, T. Coculture System with an Organotypic Brain Slice and 3D Spheroid of Carcinoma Cells. J. Vis. Exp. (80), e50881, doi:10.3791/50881 (2013).

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