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Производство и очистка нерепликативной собак аденовируса типа 2 Производные векторов
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Production and Purification of Non Replicative Canine Adenovirus Type 2 Derived Vectors

Производство и очистка нерепликативной собак аденовируса типа 2 Производные векторов

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14:55 min

December 03, 2013

DOI:

14:55 min
December 03, 2013

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Summary

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These past 15 years, canine adenovirus type 2 (CAV2)-derived vectors have proven their efficiency to transduce cells in vitro and in vivo and are widely used for vaccination and gene therapy. Here, we describe a procedure to construct, produce and purify CAV2 vectors, giving rise to high-titer viral suspensions.

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