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Production and Purification of Non Replicative Canine Adenovirus Type 2 Derived Vectors
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Kapitel
- 00:05Titel
- 01:40Transfection of Recombinant CAV2 Genomic Plasmid onto DK-E1 Cells
- 03:25Propagation of Recombinant CAV2
- 05:48Large-scale CAV2 Purification
- 10:07Titration of CAV2 by End-point Dilution
- 11:04Results: Production, Purification, and Application of CAV2
- 14:04Conclusion
These past 15 years, canine adenovirus type 2 (CAV2)-derived vectors have proven their efficiency to transduce cells in vitro and in vivo and are widely used for vaccination and gene therapy. Here, we describe a procedure to construct, produce and purify CAV2 vectors, giving rise to high-titer viral suspensions.
